Çiçek Soğanlı Bitkilerden İzole Edilmiş Fusarium oxysporum İzolatlarına Karşı Gliocladium roseum'un Etkinliği.

Abstract

The development and conservation of bulbs ornamental plants is important for both flora and economic activities. One of the most important plants is Tulip (Tulipa L.). Many of these plants are threatened by Fusarium oxysporum Schlecht, which a soil and tuber fungus. It is difficult to control after pathogen contaminate to tubers and plants. However, it is necessary to evaluate all methods of control, including biological control. Today, many biological control agents are used to control plant disease. Among these, Gliocladium spp.de is considered important. Gliocladium roseum is a filamentous fungus commonly found in soil and rotting plant residues. In general it is considered a saprophyte and produces various antibiotic substances, other fungi parasites, also stimulate plant growth. This study was carried out to determine the antagonist effect of Gliocladium roseum against Fusarium oxysporum in laboratory conditions. Fusarium oxysporum and Gliocladium roseum (Gr1, Gr2 and Gr3) isolates used in this study were obtained from tubers, plant roots and the soil. After the pathogenicity, three isolates of the Fusarium oxysporum were selected (Fo1, Fo2 and Fo3). The experiment was carried out in petri dishes containing a PDA (Potato dextrose agar) with 9 cm diameter. the pathogen and antagonist cultures developed for one week then Discs of 5 mm diameter were removed and left in the PDA medium by keeping them mutual and same distance. The growth diameters of antagonists and pathogens were measured after developing for 10 days at 22 ± 24 ° C in the incubator and the inhibition rate was calculated acording to this formula, (RI) = (R1-R2) x 100 / R1 (RI: inhibition ratio, R1: Growth radius of the pathogen, R2: It was calculated by the growth radius of the pathogen antagonist direction). As a result of the evaluation, Gliocladium roseum isolates prevented Fusarium oxysporum isolates in different ratios between 55.8-76.4 %.