The Evaluation of the Genotoxic and Cytotoxic Effects of Pyriproxyfen Insecticide on Allium cepa

Abstract

In this study, the genotoxic and cytotoxic effects of pyriproxyfen insecticide was evaluated by investigating mitotic index and phase, chromosomal abnormalities, and micronucleus proportion on root tip cells of Allium cepa L. The root types were applied to 0.25, 0.5, 1 and 2 ppm test solutions within 12, 24 and 36 h. Mitotic index was markedly lessened with increasing pyriproxyfen in each treatment group as compared to the controls. The percentages of mitotic phases have been meaningfully impacted. Pyriproxyfen noticeably heightened the anomaly cell ratio in most of the utilized solutions and application periods when compare to control. Mitotic abnormalities were determined as sticky, disturbed prophase, c-mitosis, chromatid bridges and laggards. Furthermore, micronucleus frequency was calculated at interphase.