In Vitro Propagation of Weiroot 158.

Abstract

Clonal rootstocks are commonly preferred due to their abilities to obtain early fructification and maximum yield per unit area. In vitro techniques enable rapid and intensive propagation of rootstocks in laboratory conditions. In this study, propagation possibility of Weiroot 158, an important clonal rootstock used in cherry cultivation, was investigated using tissue culture technique. Shoot tips were used as explants and Murashige-Skoog (MS) was used as the nutrient media (30 g L-1 sucrose and 7 g L-1 agar). The shoot tips were placed in the temperature and light controlled culture room after planting in the nutrient medium. Samples were taken for subculture once in a month. Number of explants, leaves and sprouting were measured every 20 days. Four different MS media were tested during the shoot multiplication phase. The best shoot proliferation was obtained in a media supplemented with 4.4 μM BAP+0.49 μM IBA+0.29 μM GA3. In addition, five different MS media were tested during the rooting phase. The results showed that the best rooting was obtained in nutrient media containing 1/2 MS + 2 mg/L IBA.