Katı Faz Fermantasyon Tekniği ile Bacillus circulans ATCC 4516’dan Ekstrasellüler β- Galaktosidaz Üretimi

Abstract

Although most microbial enzymes are produced by submerged fermentation, the use of agricultural wastes as substrates in solid state fermentation (SSF) makes the production of enzymes more economical. The use of economic substrates is of great interest for making the enzyme production process significantly cheaper for SFF. The aim of this study was to investigate the effect of some parameters on the production of β-galactosidase from Bacillus circulans ATCC 4516 using rice bran as substrate in solid state fermentation (SFF) method. Certain fermentation parameters involving incubation time, incubation temperature, inoculum level and initial pH were studied separately. Maximal amount of β- galactosidase production was obtained at 35% inoculum level, at initial pH of 7.5, at 37ºC over 48 h. In addition, various carbon and nitrogen sources were added to fermentation medium and the effect on β-galactosidase production was investigated. According to the results, carbon and nitrogen sources which added to the environment suppressed the enzyme production.