Investigation of The Antimitotic and Antimutagenic Effects of Methanolic Extracts of Pyracantha coccinea

Abstract

In this investigation, the antimitotic and antimutagenic effects of Pyracantha coccinea methanol extracts on somatic cells of Allium cepa was evaluated. The onion bulbs were applied to 400, 800, 1600, and 3200 µg /mL doses of the extract for 6, 12 and 24 h. Distilled water was utilized as a negative control and ethylmethanesulfonate was utilized as a positive control. The mitotic index mostly declined as increasing the extract concentrations. The mitotic abnormalities were observed as disturbed prophase, stickiness, laggards, chromatid bridges and c-mitosis. Also, micronucleus frequency was calculated in interphase. Thence, the antimitotic and antimutagenic potency of P. coccinea methanol extract with various tests were evaluated by using the root tip cells of A. cepa, and the use of a defined antimutagenic dose was proposed.