EFFECT OF CARBON TETRACHLORIDE (CCI4) AND ELLAGIC ACID ON RAT ERYTHROCYTE G6PD, 6PGD, GR, GST AND TrxR ENZYME ACTIVITIES

Abstract

In presented study, in vivo effects of the important polyphenol ellagic acid and CCl4 compound known for its toxic effects on metabolic activity levels of some rat erythrocyte enzymes such as G6PD (glucose 6-phosphate dehydrogenase), 6PGD (6-phosphogluconate dehydrogenase), GR (Glutathione reductase), TrxR (Thioredoxin reductase) and GST (Glutathione S-transferase) were investigated. G6PD enzyme activity meaning rised in CCl4 group and ellagic acid+CCI4 group compared to the control group (p<0.001). 6PGD enzyme activity decreased in CCl4 applyed group compared to control group (p<0.05). The enzyme activity level increased in ellagic acid (p<0.05) and CCl4+ellagic acid (p<0.01) applied groups. An increase was observed in ellagic acid (p<0.001) and CCl4+ellagic acid (p<0.001) applied groups compared to CCl4 administered group. GST enzyme activity reduced in CCI4 applied group compared to control group (p<0.01). GR enzyme activity decreased significantly in CCl4 and ellagic acid groups compared to control group (p<0.05 and p<0.01). TrxR enzyme activity decreased in CCl4 group and ellagic acid administered group compared to control group (p<0.001). A decrease in CCI4+ellagic acid group compared to control was also observed (p<0,001).