Isolation of Some Bacterial Agents from Sheep and Goat Pneumonic Lung Samples and Determination of Antimicrobial Susceptibility

Abstract

Pneumonia cases in sheep and goats lead to significant economic losses around the world. The aim of this study is to isolate and identify Pasteurella multocida, Mannheimia haemolytica, Escherichia coli, Klebsiella pneumoniae, Histophilus somni and Trueperella pyogenes as well as to determine their antimicrobial susceptibilities in lung samples of sheep and goats that were delivered to the Siirt Municipal slaughterhouse and were detected to have pneumonic lesions in their lungs after slaughter. In the study, 429 lung samples were collected from 270 goats and 159 sheep slaughtered in the Siirt Municipal Slaughterhouse. P. multocida was identified in 8 (1.86%) out of the lung samples, M. haemolytica in 8 (1.86%), E. coli in 10 (2.33%), T. pyogenes in 2 (0.46%), and Klebsiella spp. in 1 (0.23%), while H. somni was not identified. According to antimicrobial susceptibility test results, it was determined that all P. multocida and M. haemolytica isolates were sensitive to cefpodoxime, ceftiofur, enrofloxacin, florfenicol, and spectinomycin; all T. pyogenes isolates were determined to be susceptible to danofloxacin, amoxicillin, clavulanic acid, oxytetracycline, erythromycin, ceftiofur, florfenicol, sulfamethoxazole/trimethoprim; and all E. coli isolates were susceptible to ertapenem, piperacillin/tazobactam, and cefoxitin. Consequently, it was determined that especially P. multocida, M. haemolytica and E. coli strains may cause pneumonia cases in sheep and goats raised in the Siirt region. Furthermore, the isolated strains were generally susceptible to antibiotics as a result of antimicrobial susceptibility tests.