Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats

Abstract

In this study, the biochemical and histopathological changes in the lungs due to lower extremity ischemiareperfusioninjury and protective effect of boric acid to prevent them were investigated. The rats were divided into threegroups as Group 1 (Sham group, n=9), Group 2 (Ischemia-reperfusion group, I/R, n=9) and Group 3 (Ischemiareperfusion,(I/R) + boric acid (BA), n=9). Anaesthesia was applied to the first group of rats without any ischemiareperfusionprocess. Following a two-hour ischemia, two hours of reperfusion, with the help of left lower extremitiestourniquet, were applied to the second group. To the third group, 200 mg/kg (i.p.) boric acid application was performed10 minutes before the ischemia was initiated and two hours of ischemia and two hours of reperfusion application werecarried out. Some antioxidant enzymes in lung tissue (SOD, GSH-Px, CAT) were analyzed. In addition the lung tissuewas evaluated histopathologically. Boric acid application was found to significantly enhance the positive effect of antioxidantenzymes. Normal histological structure was better preserved, some of macrophages were non-activated, and aremarkable reduction of neutrophil infiltration was seen after treatment in boric acid given group.