Investigation of In Vivo Effects of Carbon Tetrachloride (CCl4) and Quercetin on Some Metabolic Enzyme Activities in Rat Erythrocyte

Abstract

In the study; the purpose was to investigate the in vivoimpact of carbon tetrachloride (CCl4)andquercetin(Qu)onactivitiesofimportantmetabolicenzymessuchasGlucose6-phosphatedehydrogenase(G6PD),6-phosphogluconatedehydrogenase(6PGD),glutathione reductase(GR)andglutathioneS-transferase(GST)inraterythrocytes. Attheexperimental stage,ratsweredividedinto4groups.1.Group(Control):Pureoliveoilatadosedetermined according to their body weight (1mL/kg) was given to the rats in this group, 2.Group (CCl4: 1.0 mL/kg(ip)(1:1),3.Group(Ku:25)mg/kg(ip),4.Group(CCl4(1.0ml/kg(ip)+Ku(25mg/kg (ip) was injected. The study was continued for 3 days.The results revealed that the activities of; G6PD (p?0.01),6PGD(p?0.01),GR(p?0.001)andGST(p?0.05)enzymeactivitieswere decreasedintheCCl4groupcomparedtothecontrolgroup.Itwasdeterminedthatenzyme activitieswerehigher in CCl4+Qu applied groups compared to CCl4group.Theapplication of Qu caused an increase in theenzyme activity value. This can be accepted as an indication that theinhibitioncausedbyCCI4hasdisappeared.Consequently;ItisthoughtthatQumaybe effective in preventing oxidative damage due to CCl4administration.