Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions

Abstract

Whereas a very small amount of pesticides which are used for elimination of undesirable speciesand for a more fertile agriculture reach the target organism, majority of pesticides reach to nontarget organisms. It isforeseen by our team that the pesticides used for various purposes may negatively affect the glutathione mechanismof the organisms. However, determination of effective dosage range is the objective of our study subjects. For thisreason, the in vitro effects of widely used pesticides (lambda-cyhalothrin, cypermethrin, chlorpyrifos, dichlorvos,glyphosate isopropylamine) on the activities of glutathione reductase (GR) and glutathione S-transferase(GST) which are two important enzymes of the glutathione system have been investigated in this study. It hasbeen determined that when lambda-cyhalothrin does not affect the GR enzyme purified from the turkey liver,chlorpyrifos, glyphosate isopropylamine, dichlorvos and cypermethrin cause inhibition. When the effects ofpesticides on GST enzyme purified from turkey liver were investigated in in vitro conditions, it was determinedthat all examined pesticides had inhibitory effects. In this context, the potential doses which can create a risk forlive life, of pesticides that are commonly used, have been identified.