Investigation of in vitro Biological Activities of 2-((6-methylpyridin-2-yl)amino)acetic acid

Abstract

Peptoids (N-substituted glycine oligomers) are a class of synthetic heteropolymers that can mimic the structures of peptides. Due to their potent biological activitiestheir use as drugs in the medical and health sectors is being investigated. This study investigates the in vitro biological activities and drug potential of the peptoid derivative 2-((6-methylpyridin-2-yl)amino)acetic acid. Antioxidant properties were determined by CUPRAC (Cupric Reducing Antioxidant Capacity)DPPH (22-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) methods. DNA protection and DNA damage analyses were performed to determine its effects on plasmid DNA. Finallyit’s in vitro inhibitory activity on the enzyme activities of GST (glutathione s-transferase) and hCA I-II (human carbonic anhydrase I-II)important enzymes of various metabolic pathwayswas determined. It was found that the highest antioxidant activity of 2-((6-methylpyridin-2-yl)amino) acetic acid was 0.0372±0.0018 µg trolox eq./µL by the CUPRAC method. While the compound did not cause any damage to DNA in the 0.5-1 mg/mL rangeit was found to have a DNA protective effect at a concentration of 0.5 mg/mL. Besidesthe best inhibitory effect of the compound was shown to be against hCA I isoenzyme.