Effect of PGPB on Germination and Early Development Parameters of Acacia cyanophylla (Lindley) Seeds in Lead Contaminated Media

Abstract

Acacia cyanopylla (Lindley) is a member of the Fabaceae family and is a decorative plant species with its yellow flowers and adds value to the place where it is used with its aesthetic beauty. In addition to its aesthetic value, it is used in sand dune prevention and afforestation works on highways. In addition to using various methods for germinating seeds, different processes are being developed using the priming technique. In this study, where the germination ability of this plant species, especially used in landscape areas, against lead pollution and the growth parameters in the early stages of plantlet formation were examined, a different approach was taken with the use of Plant Growth Promoting Bacteria (PGPB). This study was carried out in the laboratory under aseptic conditions. Brevibacterium frigoritolerans (KF58B) and Bacillus zhangzhouensis (KF3A) PGPBs were inoculated into A. cyanopylla (Lindley) seeds. Some parameters were examined by applying 100 ppm lead (Pb) to the petri dishes in which the seeds were sown. The highest germination rate and speed and shoot length were obtained as 73.3%, 33.3 and 2.57 cm, respectively, in seeds under lead stress. While root length and root thickness were obtained from the control group seeds as 3.25 cm and 0. 34 cm, respectively, the highest lateral root number, 1.07, was determined in the seeds inoculated with Brevibacterium frigoritolerans bacteria. Although the results obtained in the examined parameters differed in numerical values between applications, only the differences between the average values of shoot length, root length and root thickness were statistically.