Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system

Abstract

In the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate- N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH)2-Cu(II)) has been synthesized by radical polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with nonimprinted cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum amount of binding NANA has found as 83.20 mgg−1 at pH 7.0 with flow rate of 1 mLmin−1 at 25°C. Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient (k’) has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been studied and resulted successfully.