Development of an Efficient In Vitro Micropropagation and Microrooting Protocol from Half-Cotyledon Explants of Common Vetch (Vicia sativa L.)

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dc.contributor.authorAğamirzaoğlu
dc.contributor.authorMuhsin
dc.contributor.authorKendır
dc.contributor.authorHayrettın
dc.date.accessioned2026-10-23T18:09:51Z
dc.date.available2026-10-23T18:09:51Z
dc.date.issued2026
dc.departmentSiirt Üniversitesi
dc.description.abstractCommon vetch ( Vicia sativa L.) is an important forage legume; however
dc.description.abstractthe lack of an efficient in vitro regeneration and microrooting protocol limits its utilization in rapid propagation and future breeding studies. The aim of this study was to develop an efficient in vitro micropropagation and microrooting protocol for the common vetch cultivar Kubilay 82 using abaxially cultured half-cotyledon explants. Seed surface sterilization was evaluated using commercial sodium hypochlorite solutions at six concentrations (10
dc.description.abstract20
dc.description.abstract40
dc.description.abstract60
dc.description.abstract80 and 100%) for three exposure periods (10
dc.description.abstract15 and 20 minutes). Shoot regeneration was investigated on Murashige and Skoog (MS) medium supplemented with different combinations of 6- benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA)
dc.description.abstractwhile regenerated shoots were rooted on MS medium containing 0.75 mg L⁻¹ indole-3-butyric acid (IBA). Seed sterilization was successfully achieved under all treatments without microbial contamination
dc.description.abstractand seed germination reached 100% under several sterilization combinations. Callus induction varied between 53.33 and 86.66%
dc.description.abstractwhereas shoot induction ranged from 26.66 to 80.00%. The highest shoot induction (80.00%) and the greatest number of shoots per explant (13.66) were obtained on MS medium containing 0.20 mg L⁻¹ BAP without NAA. Microrooting percentage ranged from 60.00 to 86.66%
dc.description.abstractand the longest roots (9.96 cm) were obtained from shoots regenerated on medium containing 0.80 mg L⁻¹ BAP. The results demonstrated that BAP alone was more effective than BAP combined with NAA for shoot regeneration
dc.description.abstractwhile regenerated shoots exhibited high rooting capacity on IBA-containing medium. The developed protocol provides an efficient and reproducible regeneration and microrooting system for common vetch and represents a useful platform for future clonal propagation
dc.description.abstractgenetic improvement
dc.description.abstractand biotechnology-based breeding studies.
dc.identifier.doi10.19159/tutad.1835940
dc.identifier.endpage90
dc.identifier.issn2148-2306
dc.identifier.issn2528-858X
dc.identifier.issue2
dc.identifier.startpage82
dc.identifier.trdizinid1449973
dc.identifier.urihttps://doi.org/10.19159/tutad.1835940
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1449973
dc.identifier.urihttps://hdl.handle.net/20.500.12604/11750
dc.identifier.volume13
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürkiye Tarımsal Araştırmalar Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR_20261017
dc.subjectBitki Bilimleri
dc.titleDevelopment of an Efficient In Vitro Micropropagation and Microrooting Protocol from Half-Cotyledon Explants of Common Vetch (Vicia sativa L.)
dc.typeArticle

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