The antioxidant capacity, total phenolic content and phenolic compounds of Spergularia rubra (L.) J. Presl & C. Presl in naturally distributed five different saline areas in Siirt province

Abstract

Spergularia rubra has a very wide spread in the world. Infusion of S. rubra is used for diuretic purposes. Although widely used, the antioxidant activity of this species has not been evaluated and its chemical composition is not known [1]. In this study, antioxidant activity (DPPH %), total phenolic content (Folin Ciocalteau method) and phenolic compounds profile (LC-MS/MS) of methanolic extract of Spergularia rubra plant that collected from five different localities in Siirt were analyzed. According to the results of the analysis, the total phenolic content range from 4.33 to 13.16 mg gallic acid/mL extract. The highest antioxidant activity value was found as 77.70 %, on the other hand, the lowest was found as 57.32 %. As a result of the LC- MS/MS analysis for Cöl (Bayraktepe), location, 18 phenolic compounds were quantitatively and qualitatively determined, among the 27 phenolic compounds. The all values obtained (μg analyte/kg extract) were as follows; Quinic acid (4788.53±229.85), Malic acid (9618.56±509.78), tr-Aconitic acid (96.76±4.74), Gallic acid (141.54±7.22), Protocatechuic acid (56.17±2.86), Tannic acid (6068.41±309.49), tr-Coffeic acid (2.55±0.13), Vanilin (30.02±1.47), p-Coumaric acid (48.64±2.48), Rutin (1.13±0.05), Hyperoside (4.47±0.2), 4-OH benzoic acid (9.26±0.4), Salicylic acid (13.3±0.6) Kaempferol (63.68±3.31), Naringenin (2.33±0.12), Luteolin (35.12±2.42) Apigenin (35.14±1.86) and Rhamnetin (29.28±1.78).