Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation

Abstract

SummaryThe bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular α-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37°C. Eff ects of various carbon, nitrogen, amino acid sources and chemicals on α-amylase production were examined. FeSO4, ZnSO4 and CuSO4 inhibited bacterial growth as a result, amylase production. Maximum α-amylase production (858.6±41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.