In vitro effects of 2, 4-Dichlorophenoxy acetic acid dimethylamine salt and enrofloxacin on BTH

Abstract

In this study, the in vitro effects of enrofloxacin, a broad spectrum antibiotic, and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt that, a herbicide, on the bovine testicular hyaluronidase (BTH) were analyzed. BTH was purified using the method of ammonium sulfate precipitation and using affinity gel (Sepharose-4B-L-tyrosine-m-anisidine). Then the inhibiting effects of enrofloxacin and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt on the purified BTH enzyme were identified. IC50 values of these chemical substances were found to be 0.3101 and 0.4276 mM, respectively.