A Significant Need in the Medical Field: A New Method for Putrescine, Spermine and Spermidine Using High-Performance Liquid Chromatography (HPLC)

Abstract

The analysis of polyamines such as putrescine, spermine, and spermidine is very important for the diagnosis and treatment of various diseases, especially in the field of medicine and health. However, HPLC analysis methods are very limited in the literature. This study is very important in terms of validation parameters such as the linearity, reproducibility, and standard operating curves of polyamines such as serum putrescine, spermine and spermidine. Therefore, we developed a modified method for the analysis of serum putrescine, spermine, and spermidine. This study is superior to indirect measurement methods and is important as a gold standard. In this study, a reproducible method was developed according to the standard putrescine, spermine, and spermidine working curves and other validation criteria for HPLC. Polyamine biosynthesis is an ancient metabolic pathway found in all living organisms and the homeostasis of polyamines is essential for cell survival. This new HPLC method is a first in the field of medicine in that it provides fast, accurate, and reliable results in serum putrescine, spermine, and spermidine analysis and is used in routine analyses in the diagnosis of some specific diseases.