Effects of exogenous fibrolytic enzymes supplementation on growth of Pontastacus leptodactylus performance and ruminal fermentation in pre-weaning Simmental calves

Early adaptation of newborn calves' forestomaches concentrates and roughageiscrucial for cost-effectiveness. ABSRACT Therefore, the use of additives that will facilitate early adaptation to feed and positively impact forestomach methoate DM ) pes c ide s one of t e chem cals used to developmentiscrucial.Thisstudyaimedtodeterminethe otectsomeagriculturalareafromharmful oganismsDMT effects of exogenous fibrolytic enzyme supplementation on siduesreeaseddirectlyorindirectlyotheenvironment airsgrowth performance and rumen fermentation in pre-weaning use ser ous p oblem in nature DMT residues m ixed with the calves. Eighteen Simmental male calves of the same age (4 aenronnutic vi mt avrsede yaffectauaranmtogiss d days) were randomly assigned to 3 groups and supplemented q an with exogenous fibrolytic enzyme additive, 0 (Control), 2 g.d-tiseffect iscarried to humansthrough thefoodchain. Inhis i iu y, oxidative s tre ss responses nd ced by DMT pesticide n 1, or 4 g,d(-1) for 84 days treatments included with exogenous ontastaculeptodactylusweenvestigatedForthspurose fibrolytic enzyme additive, 0(Control), 2 g.d(-1) or 4 g.d(-1) The exogenous fibrolytic enzyme asupplementation to calves idat ve stress a nd antioxi dant par meters Thiobarbi ur c acidsignificantly improved feed conversionratio(P<0.01).Ruminal activesubsances(TBARS)Glutathione(GSH)Superoxide ismutase (SOD)ctalase(CAT)andglutathioneperoxdase pH and ammonia nitrogen (NH3-N concentrations were not affected by exogenous fibrolytic enzyme supplementation on sPX) cau edbydimethoate(DMT)pestcideinPleptodactyus days 42 and 84 of the study (P>0.05). Ruminal concentrations of acetic acid, propionic acid, and butyric acid were not affected 17 5, 35, and 70 mgL 1 concentrations at 24 and 96 hours Eereinvestigated.Resultswere determined using LISAkis by exogenous fibrolytic enzyme supplementation on day 42 iof the study (P>0.05). ii i higher in both exogenous fibrolytic enzyme-supplemented ereobservedbetweendecreasesnCATandGPxactivitiesand groups than in the Control group on the 84th day of the study creasesinTBARSlevesSPSS240packageprogramone-wa (P<0.01). The butyric acid concentration at 2 g.d(-1 )exogeno l iNOVA (Duncan 0 05) was used in the eva uat on of biochem cal fibrolytic enzyme supplemented group was higher than the other groups on the 84th day of the study (P<0.001). The acetic acid to propionic acid ratio was higher in the Control as e uwaeasoxatve lyze As a r lt, it s d termined th t DMT cau ed idi ressformationinP.leptodactylusandcaued changes inenzyme and 2 g.d(-1) exogenous fibrolytic enzyme-supplemented groups than inthe 4 g.d(-1) exogenous fibrolytic enzyme-supplemented tivities : igroup on the 42nd day of the study (P<0.05). The acetic acid ey wordsDimethoate,Pontastacus leptodactylus,oxdative to propionic acid ratio was higher in the Control group than in the exogenous fibrolytic enzyme-supplemented groups on day stress, antioxidant, biomarkers84 of the study (P<0.01). The results indicated that 2 or 4 g.d(-1) exogenous fibrolytic enzyme su feed conversion ratio and ruminal propionic concentration in pre-weaning calves. to . The propionic acid concentration was us pplementation had a better