Cost-effective immobilization of Chaetomium erraticum dextranase on c-MWCNT

Abstract

In this study, dextranase enzyme (CED) from Chaetomium erraticum was immobilized on carboxylated multiwalled carbon nanotubes (c-MWCNTs) via adsorption. Immobilization conditions were optimized, and both free (FCED) and immobilized enzymes (ICED) were characterized. The process achieved 100 % immobilization efficiency and 114.13 % activity recovery. After immobilization, the optimum pH shifted from 5.0 to 6.0, while the optimum temperature remained constant at 55 degrees C. The Km value was identical for both enzymes (54.35 g/L), but ICED exhibited a higher Vmax than FCED. ICED retained its activity after 20 reuses and 30 days of storage. Moreover, isomaltooligosaccharide (IMO) production by ICED was completed in 10 h. The results demonstrate that this approach enables simple, rapid, and cost-effective enzyme immobilization suitable for industrial IMO production.