Using rice anther culture for the production of doubled haploid lines with increased induction frequencies

Basit Öğe Kaydı
dc.contributor.authorSharma, Manmohan
dc.contributor.authorSharma, Mamta
dc.contributor.authorLone, Javed Ahmad
dc.contributor.authorShikari, Asif B.
dc.contributor.authorPandey, Saurabh
dc.contributor.authorEl-Badri, Ali Mahmoud
dc.contributor.authorSalgotra, R. K.
dc.date.accessioned2026-10-23T18:13:41Z
dc.date.available2026-10-23T18:13:41Z
dc.date.issued2026
dc.departmentSiirt Üniversitesi
dc.description.abstractDeveloping doubled haploid (DH) lines through anther or microspore culture is an important strategy for shortening breeding cycles and accelerating crop improvement. The present study aimed to enhance the regeneration of rice haploids through anther culture-induced calli and subsequently convert them into DH bacterial leaf blight (BLB)-resistant lines. Eleven lines expressing resistance against prevalent strains of Xanthomonas oryzae pv. oryzae were crossed as male parents with elite high-yielding basmati and non-Basmati varieties to develop F(1)s for collecting anthers at the appropriate stage of pollen development. Optimum callus induction in 36.45% of the genotypes was obtained on N-6 medium supplemented with maltose (40 g/l), 2,4-D (2.5 mg/l), and kinetin (0.5 mg/l), and further callus proliferation was enhanced in the presence of amino acids, particularly a combination of cysteine and tryptophan. High scores of haploid regeneration frequency were obtained in MS medium augmented with sucrose (30 g/L), BAP (2.0 mg/l), kinetin (0.5 mg/l), and NAA (0.5 mg/l). Fortification of regeneration medium with tryptophan (25 mg/l) and cysteine (40 mg/l) resulted in maximum regeneration of calli. Ranbir Basmati, Saanwal Basmati, Basmati 564, Ranbir Basmati & times; IRBB53, Ranbir Basmati & times; IRBB60, Basmati 564 & times; IRBB57, and Basmati 370 & times; IRBB56 were observed to be the most responsive genotypes for haploid regeneration. An average of 76.34% DH induction frequencies were obtained in colchicine (0.1%) treatment, while 41.56% were spontaneously induced DHs. Consequently, using anther culture to develop DH lines is a promising approach for genomic resources and elite varieties in a shorter breeding period.
dc.description.sponsorshipDeanship of Graduate Studies and Scientific Research, Taif University, Taif -- The authors thank the Deanship of Graduate Studies and Scientific Research, Taif University, Taif, for funding the present study.
dc.identifier.doi10.18520/cs/v130/i10/914-925
dc.identifier.endpage925
dc.identifier.issn0011-3891
dc.identifier.issue10
dc.identifier.scopus2-s2.0-105040358472
dc.identifier.scopusqualityQ2
dc.identifier.startpage914
dc.identifier.urihttps://doi.org/10.18520/cs/v130/i10/914-925
dc.identifier.urihttps://hdl.handle.net/20.500.12604/12087
dc.identifier.volume130
dc.identifier.wosWOS:001780232000009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherIndian Acad Sciences
dc.relation.ispartofCurrent Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20261017
dc.subjectAnther Culture
dc.subjectBacterial Leaf Blight
dc.subjectCallus
dc.subjectDoubled Haploids
dc.subjectHaploids
dc.subjectRice
dc.titleUsing rice anther culture for the production of doubled haploid lines with increased induction frequencies
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu